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KMID : 0368419860290030145
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Journal of Plant Biology
1986 Volume.29 No. 3 p.145 ~ p.156
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A Comparative Study on the Nitrogen Metabolism of Symbiotic Chlorella from Paramecium bursaria with Chlorella ellipsoidea
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Chang Nam-Kee
Ha Kwon-Soo
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Abstract
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The excretion of ammonia and glutamine synthetase activities were measured in aposymbiotic Paramecium and symbiotic Paramecium. The uptake of nitrate and ammonia, and specific enzyme activities of nitrate reductase, glutamate dehydrogenase and glutamine synthetase were investigated in symbiotic Chlorella from Paramecium bursaria and Chlorella ellipsoidea.
The ammonia concentration in the culture media of aposymbiotic Paramecium was increased according to the growth of the Paramecium but it was not changed in symbiotic Paramecium. Nitrate, the major nitrogen source, was taken up at a rate of 0.635 μmol/10^5 Chlorella/hr in Chlorella ellipsoidea. Most of ammonia was assimilated to glutamine by glutamine synthetase, fo which activity was 1,467 u㏖/㎎ protein/min in Chlorella ellipsoidea.
Contrary to Chlorella ellipsoidea, ammonia and glutamine transported from the Paramecium were the nitrogen source of symbiotic Chlorella and ammonia was taken up at a rate of 3.854 n㏖/10^5 Chlorella/hr into symbiotic Chlorella. Most of ammonia were assimilated to glutamate by glutamate dehydrogenase in symbiotic Chlorella. The glutamate dehydrogenase (GDH/NADH) activity was 0.851 μ㏖/㎎ protein/min.
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